Top latest Five attune flow cytometer Urban news
Top latest Five attune flow cytometer Urban news
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Knowledge-enriched characterization of cell populations. Morphological information and facts from photos can incorporate towards the richness of flow cytometry information. By way of example, the figure exhibits an or else conventional apoptosis assay using Annexin V and PI, including mobile imaging to characterize cells in Each and every populace to expose morphologically unique characteristics. These insights couldn't are actually attained from multiplex staining by itself.
Whether you’re a novice or a specialist, take a look at our means to advance your study and knowledge in flow cytometry.
(B) Uncooked 264.seven macrophages will often be annotated inaccurately with respect to particle depend because of their complexity and noncircular borders. The amount of singlets readily available for Examination increases when the consumer trains and employs a far more correct design.
Explore new insights into your experimental information by visualizing populations utilizing combined fluorescence and morphology data. Our software interface wizards permit downsampling, concatenation (merged data files) and dimensionality applications which are created in the program for an intuitive, streamlined data analysis working experience.
"A picture is just not fairly worthy of one thousand dots, nonetheless it definitely really helps to see images. It is excellent to find out photos that correlate with staining. For example, AnnexinV-favourable cells which might be morphologically distinct than healthful cells, or CD14+ monocytes which can be larger plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Listed here, a highly trained consumer has gated singlets confidently. Just after analyzing the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate contains a lot more than four% aggregates.
Optimizing gating approach. Even strong manual singlet gating is mistake-inclined and continues to be a subjective decision point in almost all flow cytometry assays. Imaging can be employed to verify and change gates to include only one cells of interest.
Our experts will layout panels for you personally as repeatedly you require—gratis, no product or service obtain essential.
The Attune NxT cytometer is the trusted workhorse instrument your lab can rely upon for constant and trusted efficiency. The Attune CytPix design incorporates a superior-speed brightfield digicam and AI-pushed impression Assessment software program, with recent updates such as equipment Studying tools and an intuitive user interface, enabling enhanced information precision, accuracy, and more quickly insights into sample biology.
Maybe most significantly, these activities incorporate cells of Plainly distinctive phenotypes which may lead to incorrect conclusions pertaining to double constructive occasions (particularly in unusual populations).
AI-driven automated picture analysis While using the Attune Cytometric Software package interprets function functions into distinctive morphology parameters that can be coupled with common fluorescence and scatter parameters to expose hugely reproducible inhabitants variations and exclusive subsets.
We Earlier shown the power of imaging Car or truck-T/Ramos cell interactions. Permit’s have a look at just the inhabitants of best curiosity, the double positive events, to learn more. We can now use extended morphological parameters (here, circularity and skewness of intensity) to more take a look at the functions of those populations and refine gating on these occasions, raising details robustness.
Leverage the power of Sophisticated technologies and innovation to speed up your discoveries. Rely on in the Attune Flow Cytometer relatives to provide the dependability, pace, and precision that set your perform apart.
Flexible payment program from Agilent Money Answers permit you to obtain the crucial instrumentation you may need for fast job, or to grow your lab.
Gating on these prolonged parameters enables you to quickly and precisely recognize populations of interest and ensure gating system with little or no handbook evaluate. The Attune application provides entry to condition-of-the-artwork device Studying Assessment more info instruments within just an expertly made person interface, making it exceptionally user friendly.
As the cell line experienced Earlier been analyzed over the Attune CytPix Flow Cytometer, the researchers went back again to the images and ended up able to document the microbial an infection no less than 5 days before.
The Attune CytPix Flow Cytometer permits users to visualise samples all through acquisition, confirm the accuracy of gating strategies, and support success with trustworthy documentation for publication purposes.
What seemed to be a homogeneous populace is in fact far more various—an insight that ought to be thought of when interpreting final results.